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human genome u219 microarrays  (Thermo Fisher)


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    Thermo Fisher human genome u219 microarrays
    Human Genome U219 Microarrays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human genome u219 microarrays/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    human genome u219 microarrays - by Bioz Stars, 2026-04
    90/100 stars

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    A+B) A genome-wide unbiased principal component analysis including 12621 genes classifies VSEL cells as remote to both pluripotent and adult stem cell populations as well as mature blood cell lineages. C) Hierarchical clustering represented by an un-rooted tree diagram confirms the results of the PCA. D) A heatmap comparison of 371 selected pluripotency associated genes shows substantial differences between VSEL cells and pluripotent stem cells, but a high degree of similarity between ES and iPS cells. E) Normalized transcription level of the core pluripotency genes Oct4 (POU5F1), Nanog and Sox2 as part of D. Note that the Affymetrix microarray probes for POU5F1 do not comply with up to date quality standards as they are known to detect pseudogenes.

    Journal: PLoS ONE

    Article Title: Very Small Embryonic-Like Stem Cells Purified from Umbilical Cord Blood Lack Stem Cell Characteristics

    doi: 10.1371/journal.pone.0034899

    Figure Lengend Snippet: A+B) A genome-wide unbiased principal component analysis including 12621 genes classifies VSEL cells as remote to both pluripotent and adult stem cell populations as well as mature blood cell lineages. C) Hierarchical clustering represented by an un-rooted tree diagram confirms the results of the PCA. D) A heatmap comparison of 371 selected pluripotency associated genes shows substantial differences between VSEL cells and pluripotent stem cells, but a high degree of similarity between ES and iPS cells. E) Normalized transcription level of the core pluripotency genes Oct4 (POU5F1), Nanog and Sox2 as part of D. Note that the Affymetrix microarray probes for POU5F1 do not comply with up to date quality standards as they are known to detect pseudogenes.

    Article Snippet: RNA extraction, labelling, hybridisation and microarray analysis was conducted by AROS Applied Biotechnology AS (Aarhus, Denmark) using Rneasy kit (Qiagen, Hilden, Germany), NuGen Pico labelling kit (NuGen, San Carlos, USA) and Affymetrix GeneTitan Human Genome U219 microarray (Affymetrix, Santa Clara, USA) according to in-house protocols.

    Techniques: Genome Wide, Microarray